Publication List

→Before 2002

Selected Paper Article

Inoue R, Harada K, Wakayama S, Ooga M, Wakayama T.　Improvement of a twice collection method of mouse oocytes by surgical operation. J Reprod Dev. 2020 Oct 13;66(5):427-433 doi: 10.1262/jrd.2020-059. Online ahead of print.

Konno S, Wakayama S, Ito D, Kazama K, Hirose N, Ooga M, Wakayama T.　Removal of remodeling/reprogramming factors from oocytes and the impact on the full-term development of cloned embryos.  Development. 2020 Aug 7;147(15):dev190777. doi: 10.1242/dev.190777.

Hirose N, Wakayama S, Inoue R, Ito J, Ooga M, Wakayama T. Birth of offspring from spermatid or somatic cell by co-injection of PLCζ-cRNA. Reproduction. 2020 Aug;160(2):319-330. doi: 10.1530/REP-20-0054.

Yamamoto Y, Hirose N, Kamimura S, Wakayama S, Ito J, Ooga M, Wakayama T. Production of mouse offspring from inactivated spermatozoa using horse PLCζ mRNA. J Reprod Dev. 2020 Feb 14;66(1):67-73. doi: 10.1262/jrd.2019-043. Epub 2019 Dec 18.

Araki R,  Hoki Y,  Suga T, Obara C, Sunayama M, Imadome K, Fujita M, Kamimura S, Nakamura M, Wakayama S, Nagy A, Wakayama T, Abe M. Genetic aberrations in iPSCs are introduced by a transient G1-S cell cycle checkpoint deficiency. Nat Commun. 2020 Jan 10;11(1):197. doi: 10.1038/s41467-019-13830-x.

Uchikura A, Matsunari H, Maehara M, Yonamine S, Wakayama S, Wakayama T, Nagashima H. Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance. Reprod Med Biol. 2019 Dec 21; 19(2): 142-150. doi: 10.1002/rmb2.12312. eCollection 2020 Apr.

Ito D, Wakayama S, KamadaY, Shibasaki I, Kamimura S, Ooga M, Wakayama T. Effect of trehalose on the preservation of freeze-dried mice spermatozoaat room temperature. J Reprod Dev. 2019 Aug 9;65(4):353-359. doi: 10.1262/jrd.2019-058.

Wakayama S, Ito D, Kamada Y, Yonemura S, Ooga M, Kishigami S, Wakayama T. Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from -196℃ to 150℃. Sci Rep. 2019 Apr 5;9(1):5719. doi: 10.1038/s41598-019-42062-8

Wakayama S, Kishigami S, Wakayama T. Improvement of mouse cloning from any type of cell by nuclear injection. Methods Mol Biol. 2019;1874:211-228. doi: 10.1007/978-1-4939-8831-0_12.

Kamimura S, Wakayama S, Kuwayama H, Tanabe Y, Kishigami S, Wakayama T. Generation of two-cell cloned embryos from mouse faecal cell. Sci Rep. 2018 Oct 8;8(1):14922. doi: 10.1038/s41598-018-33304-2.

Kamada Y, Wakayama S, Shibasaki I, Ito D, Kamimura S, Ooga M, Wakayama T. Assessing the tolerance to room temperature and viability of freeze-dried mice spermatozoa over long-term storage at room temperature under vacuum. Sci Rep. 2018 Jul 13;8(1):10602. doi: 10.1038/s41598-018-28896-8.

Ooga M, Funaya S, Aoki F, Wakayama T. Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development. J Vis Exp. 2018 Jun 12;(136). doi: 10.3791/57068.　PMID: 29985353

Nagatomo H, Yao T, Araki Y, Mizutani E, Wakayama T. Agarose capsules as new tools for protecting denuded mouse oocytes/embryos during handling and freezing-thawing and supporting embryonic development in vivo. Sci Rep. 2017 Dec 20;7(1) :17960. doi: 10.1038/s41598-017-18365-z

Tanabe Y, Kuwayama H, Wakayama S, Nagatomo H, Ooga M, Kamimura S, Kishigami S, Wakayama T. Production of cloned mice using oocytes derived from ICR-outbred strain. Reproduction 2017 Dec;154(6):859-866. doi: 10.1530/REP-17-0372. Epub 2017 Oct 2.

Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, Wakayama T, Yamamoto T, Yamada Y. Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation. Nature 2017 Aug 10;548(7666):224-227. doi: 10.1038/nature23286. Epub 2017 Jul 26.

Ogushi S, Yamagata K, Obuse C, Furuta K, Wakayama T, Matzuk MM, Saitou M. Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2. J Cell Sci. 2017 Jul 15;130(14):2416-2429. doi: 10.1242/jcs.195875. Epub 2017 Jun 9.

Ooga M, Wakayama T. FRAP analysis of chromatin looseness in mouse zygotes that allows full-term development PLOS ONE 22 May 2017 https://doi.org/10.1371/journal.pone.0178255

Wakayama S, Kamada Y, Yamanaka K, Kohda T, Suzuki H, Shimazu T, Tada MN, Osada I, Nagamatsu A, Kamimura S, Nagatomo H, Mizutani E, Ishino F, Yano S, Wakayama T. Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months. Proc Natl Acad Sci U S A. 2017 May 22. pii: 201701425. doi: 10.1073/pnas.1701425114.

Araki R, Mizutani E, Hoki Y, Sunayama M, Wakayama S, Nagatomo H, Kasama Y, Nakamura M, Wakayama T, Abe M. The Number of Point Mutations In iPS Cells and ntES Cells Depends on the Method and Somatic Cell Type Employed for Their Generation. Stem Cells. 2017 May;35(5):1189-1196　Feb 24. doi: 10.1002/stem.2601.

Kuwayama H, Tanabe Y, Wakayama T, Kishigami S. Birth of cloned mice from vaginal smear cells after somatic cell nuclear transfer. Theriogenology. 2017 May;94:79-85. doi: 10.1016/j.theriogenology.2017.02.012. Epub 2017 Feb 20.

Wakayama S, Tanabe Y, Nagatomo H, Mizutani E, Kishigami S, Wakayama T. Effect of Long-Term Exposure of Donor Nuclei to the Oocyte Cytoplasm on Production of Cloned Mice Using Serial Nuclear Transfer. Cell Reprogram. 2016 Nov;18(6):382-389.

Kim HR, Lee JE, Oqani RK, Kim SY, Wakayama T, Li C, Sa SJ, Woo JS, Jin DI. Aberrant Expression of TIMP-2 and PBEF Genes in the Placentae of Cloned Mice Due to Epigenetic Reprogramming Error. PLoS One. 2016 Nov 17;11(11):e0166241. doi: 10.1371/journal.pone.0166241. eCollection 2016

Lee AR, Shimoike T, Wakayama T, Kishigami S. Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice. Cell Reprogram. 2016 Jun;18(3):147-53. doi: 10.1089/cell.2016.0014.

Koike T, Wakai T, Jincho Y, Sakashita A, Kobayashi H, Mizutani E, Wakayama S, Miura F, Ito T, Kono T. DNA Methylation Errors in Cloned Mouse Sperm by Germ Line Barrier Evasion. Biol Reprod. 2016 Jun;94(6):128. doi: 10.1095/biolreprod.116.138677. Epub 2016 Apr 20.

Mizutani et al., Generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells. Sci Rep. 2016 Apr 1;6:23808. doi: 10.1038/srep23808.

Kumar et al.,　Antibody repertoire diversification through VH gene replacement in mice cloned from an IgA plasma cell.　Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):E450-7. doi: 10.1073/pnas.1417988112. Epub 2015 Jan 21.

Mizutani et al., Generation of Cloned Mice from Adult Neurons by Direct Nuclear Transfer.　Biol Reprod. 2015 92:81. doi: 10.1095/biolreprod.114.1234552015

Iwamoto et al., Early Development of Cloned Bovine Embryos Produced from Oocytes Enucleated by Fluorescence Metaphase II Imaging Using a Conventional Halogen-Lamp Microscope.　Cell Reprogram. 2015 Apr;17(2):106-114. doi: 10.1089/cell.2014.0086.

Ishiuchi et al.,  Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly. Nature Struct Mol Biol. 2015 Sep;22(9):662-671. doi: 10.1038/nsmb.3066.

Kyogoku et al.,　De novo formation of nucleoli in developing mouse embryos originating from enucleolated zygotes. Development. 2014 Jun; 141(11): 2255-2259. doi: 10.1242/dev.106948. Epub 2014 May 6.

Wakayama et al., Successful Serial Recloning in the Mouse over Multiple Generations.　Cell Stem Cell 2013 Mar 7;12(3):293-7. doi: 10.1016/j.stem.2013.01.005. (Cover article of issue)

Lee AR, et al.,　Nicotinamide: a Class III HDACi Delays In Vitro Aging of Mouse Oocytes.　J Reprod Dev. 2013; 59:238-244.

Terashita et al., Latrunculin A treatment prevents abnormal chromosome segregation for successful development of cloned embryos PLoS One. 2013 Oct 24;8(10):e78380. doi: 10.1371/journal.pone.0078380.

Takase et al., Nuclear transferred embryonic stem cell for analysis of B1 B-lymphocyte development. Int Immunol. 2013 Mar;25(3):145-56. doi: 10.1093/intimm/dxs095.

Ogura A, Inoue K, Wakayama T. (2013) Recent advancements in cloning by somatic cell nuclear transfer. Philos Trans R Soc Lond B Biol Sci.

Mizutani E, Ogura A, Wakayama T. (2013) Nuclear transfer in the mouse oocyte. Methods Mol Biol.

Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T. (2012) Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent. PLoS One.

Nagashimada M, Ohta H, Li C, Nakao K, Uesaka T, Brunet JF, Amiel J, Trochet D, Wakayama T, Enomoto H. (2012) Autonomic neurocristopathy-associated mutations in PHOX2B dysregulate Sox10 expression. J Clin Invest.

Terashita Y, Wakayama S, Yamagata K, Li C, Sato E, Wakayama T. (2012) Latrunculin A can improve the birth rate of cloned mice and simplify the nuclear transfer protocol by gently inhibiting actin polymerization. Biol Reprod.

Yamagata K, Iwamoto D, Terashita Y, Li C, Wakayama S, Hayashi-Takanaka Y, Kimura H, Saeki K, Wakayama T. (2012) Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy. PLoS One.

Mizutani E, Yamagata K, Ono T, Akagi S, Geshi M, Wakayama T. (2012) Abnormal chromosome segregation at early cleavage is a major cause of the full-term developmental failure of mouse clones. Dev Biol. in press

Kohda T, Kishigami S, Kaneko-Ishino T, Wakayama T, Ishino F. (2012) Gene Expression Profile Normalization in Cloned Mice by Trichostatin A Treatment. Cell Reprogram. in press

Bui HT, Seo HJ, Park MR, Park JY, Van Thuan N, Wakayama T, Kim JH. (2011) Histone Deacetylase Inhibition Improves Activation of Ribosomal RNA Genes and Embryonic Nucleolar Reprogramming in Cloned Mouse Embryos.Biol Reprod. 85:1048-56

Kohda T, Ogonuki N, Inoue K, Furuse T, Kaneda H, Suzuki T, Kaneko-Ishino T, Wakayama T, Wakana S, Ogura A, Ishino F. (2011) Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect. Biochem Biophys Res Commun. 410:282-8.

Amarnath D, Choi I, Moawad AR, Wakayama T, Campbell KH. (2011) Nuclear-cytoplasmic incompatibility and inefficient development of pig-mouse cytoplasmic hybrid embryos. Reproduction. 142:295-307.

Ono T, Mizutani E, Li C, Yamagata K, Wakayama T. (2011) Offspring from intracytoplasmic sperm injection of aged mouse oocytes treated with caffeine or MG132. Genesis. 49:460-71.

Maruya M, Suzuki K, Fujimoto H, Miyajima M, Kanagawa O, Wakayama T, Fagarasan S. (2011) Vitamin A-dependent transcriptional activation of the nuclear factor of activated T cells c1 (NFATc1) is critical for the development and survival of B1 cells. Proc. Natl. Acad. Sci. U S A 108:722-7.

Murakami K, Araki K, Ohtsuka S, Wakayama T, Niwa H. (2011) Choice of random rather than imprinted X inactivation in female embryonic stem cell-derived extra-embryonic cells. Development 138:197-202.

Bui HT, Wakayama S, Mizutani E, Park KK, Kim JH, Van Thuan N, Wakayama T. (2011)
Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction 141:67-77.

Hirata S, Fukasawa H, Wakayama S, Wakayama T, Hoshi K. (2011) Generation of Healthy Cloned Mice Using Enucleated Cryopreserved Oocytes. Cell Reprogram. 13:7-11.

Ono T, Li C, Mizutani E, Terashita Y, Yamagata K, Wakayama T. (2010) Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice. Biol. Reprod. 83:929-937.

Kim HR, Han RX, Wakayama T, Park CS, Jin DI. (2010) Aberrant protein expression in the placenta of cloned mouse derived from embryonic stem cell. Placenta 31:853-859.

Hikichi T, Ohta H, Wakayama S, Wakayama T. (2010) Functional full-term placentas formed from parthenogenetic embryos using serial nuclear transfer. Development 137:2841-2847.

Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T. (2010) Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system. Dev Biol. 346:90-101.

Sakaue M, Ohta H, Kumaki Y, Oda M, Sakaide Y, Matsuoka C, Yamagiwa A, Niwa H, Wakayama T, Okano M. (2010) DNA Methylation Is Dispensable for the Growth and Survival of the Extraembryonic Lineages. Curr. Biol. 20:1452-1457.

Bui HT, Wakayama S, Kishigami S, Park KK, Kim JH, Van Thuan N, Wakayama T. (2010) Effect of Trichostatin A on Chromatin Remodeling, Histone Modifications, DNA Replication, and Transcriptional Activity in Cloned Mouse Embryos. Biol. Reprod. 83:454-463

Sato S, Yagi S, Arai Y, Hirabayashi K, Hattori N, Iwatani M, Okita K, Ohgane J, Tanaka S, Wakayama T, Yamanaka S, Shiota K. (2010) Genome-wide DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) residing in mouse pluripotent stem cells. Genes Cells. 15:607-18

Dahl JA, Reiner AH, Klungland A, Wakayama T, Collas P. (2010) Histone H3 lysine 27 methylation asymmetry on developmentally-regulated promoters distinguish the first two lineages in mouse preimplantation embryos. PLoS One. 5: e9150.

Ono T, Mizutani E, Li C, Wakayama T. (2010) Preservation of sperm within the mouse cauda epididymidis in salt or sugars at room temperature. Zygote. 18:245-256.

Okada Y, Yamagata K, Hong K, Wakayama T, Zhang Y. (2010) A role for the elongator complex in zygotic paternal genome demethylation. Nature 28:554-558.

Li C, Mizutani E, Ono T, Wakayama T. (2010) An Efficient Method for Generating Transgenic Mice Using NaOH-treated Spermatozoa. Biol Reprod. 82: 331-40.

Ohta H, Ohinata Y, Ikawa M, Morioka Y, Sakaide Y, Saitou M, Kanagawa O, Wakatama T. (2009) Male Germline and Embryonic Stem Cell Lines from NOD Mice: Efficient Derivation of GS Cells from a Nonpermissive Strain for ES Cell Derivation. Biol Reprod. 81: 1147-53.

Wakayama S, Kawahara Y, Li C, Yamagata K, Yuge L, Wakayama T.（2009）Detrimental Effects of Microgravity on Mouse Preimplantation Development In Vitro PLoS One. e6753.

Oda M, Tanaka S, Yamazaki Y, Ohta H, Iwatani M, Suzuki M, Ohgane J, Hattori N, Yanagimachi R, Wakayama T, Shioda K. (2009) Establishment of trophoblast stem cell line from somatic cell neclear-transferred embryos. Proc. Natl. Acad. Sci. USA 106:16293-7.

Yamagata K, Suetsugu R, Wakayama T.（2009）Assessment of chromosomal integrity using a novel live-cell imaging technique in mouse embryos produced by intracytoplasmic sperm injection. Hum Reprod. 24: 2490-9

Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T.（2009）
The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice. Reproduction 138: 309-17.

Ohinata Y, Ohta H, Shigeta M, Yamanaka K, Wakayama T, Saitou M.（2009）
A signaling principle for the specification of the germ cell lineage in mice. Cell 137 :571-84.

Bui HT, Hwang KC, Kim JH, VAN Thuan N, Wakayama T, MiyanoT. （2009）Effect of Vanadate on the Chromatin Configuration in Pig GV-oocytes. J. Reprod. Dev., 55:367-72.

Yamagata K, Suetsugu R, Wakayama T. (2009) Long-term, Six-dimensional Live-cell Imaging for the Mouse Preimplantation Embryo That Does Not Affect Full-term Development. J. Reprod. Dev. 55: 343-50.

Li C, Mizutani E, Ono T, Wakayama T. (2009). Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI. Reproduction 137:779-92.

Ohta H, Sakaide Y, Wakayama T. (2009). Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI. Hum Reprod. 24:775-81.

Ohta H, Sakaide Y, Wakayama T. (2009) Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny. Biol Reprod. 80 :511-7.

Bui HT, Wakayama S, Kishigami S, Kim JH, Van Thuan N, Wakayama T. (2008) The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming. Development. 135:3935-45.

Wakayama S, Ohta H, Hikichi T, Mizutani E, Iwaki T, Kanagawa O, Wakayama T.(2008) Production of healthy cloned mice from bodies frozen at -20{degrees}C for 16 years. Proc. Natl. Acad. Sci. USA 105:17318-22.

Ono T, Mizutani E, Li C, Wakayama T. (2008) Nuclear Transfer Preserves the Nuclear Genome of Freeze-Dried Mouse Cells.
J. Reprod. Dev. 54 :486-91.

Ohta H, Sakaide Y, Wakayama T. (2008) Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages. Reproduction 136:581-587.

Ohta H, Sakaide Y, Yamagata K, Wakayama T. (2008) Increasing the cell number of host tetraploid embryos can improve the production of mice derived from embryonic stem cells. Biol. Reprod. 79:486-492.

Mizutani E, Ono T, Li Chong,  Maki-Suetsugu R, Wakayama T. (2008) Propagation of senescent mice using nuclear transfer embryonic stem cell lines. Genesis 46:478-83.

Ohta H, Sakaide Y, and Wakayama T. (2008)  Long-Term Preservation of Mouse Spermatozoa as Frozen Testicular Sections. J. Reprod. Dev. 54：295-298.

Suzuki T,　Kondo S, and Wakayama T, Cizdziel PE, and Hayashizaki Y. (2008) Genome-Wide Analysis of Abnormal H3K9 Acetylation in Cloned Mice. PLoS One 3:e1905.

Shibata S, Yokota T, and Wakayama T.(2008) X-chromosome inactivation in nuclear transfer ES cells. Cytogenetic and Genome Research 121: 96-101

Tabar V, Tomishima M, Panagiotakos G, Wakayama S, Menon J, Chan B, Mizutani E, Al-Shamy G, Ohta H, Wakayama T, and Studer L.(2008) Therapeutic cloning in individual parkinsonian mice. Nat. Med. 14: 379-381.

Ohta H, Sakaide Y, and Wakayama T. (2008) The Birth of Mice from Testicular Spermatozoa Retrieved from Frozen Testicular Sections. Biol Reprod 78: 807-811.

Hikichi T, Kohda T, Wakayama S, Ishino F, and Wakayama T. (2008)Nuclear Transfer Alters the DNA Methylation Status of Specific Genes in Fertilized And Parthenogenetically Activated Mouse Embryonic Stem Cells. Stem Cells. 26: 783-788.

Wakayama S, Kishigami S, Thuan NV, Ohta H, Hikichi T, Mizutani E, Bui HT, Miyake M, and Wakayama T. (2008)  Effect of volume of oocyte cytoplasm on embryo development after parthenogenetic activation, intracytoplasmic sperm injection, or somatic cell nuclear transfer. Zygote 16:211-222.

Kishigami S, and Wakayama T. (2007) Efficient Strontium-Induced Activation of Mouse Oocytes in Standard Culture Media by Chelating Calcium. J. Reprod. Dev. 2007 53:1207-1215

Kim K, Ng K, Rugg-Gunn PJ, Shieh JH, Kirak O, Jaenisch R, Wakayama T, Moore MA, Pedersen RA, and Daley GQ. (2007) Recombination Signatures Distinguish Embryonic Stem Cells Derived by Parthenogenesis and Somatic Cell Nuclear Transfer. Cell Stem Cells 1:346-352

Senda S, and Wakayama T, Arai Y, Yamazaki Y,  Ohgane J, Tanaka S, Hattori N, Yanagimachi R, and Shiota K. (2007)　DNA Methylation Errors in Cloned Mice Disappear with Advancement of Aging.　Cloning Stem Cells 9:293-302

Ohta H, Sakaide Y, and Wakayama T. (2007) Generation of progeny via ICSI following enrichment of elongated spermatids from mouse testis by flow-cytometric cell sorting. Hum Reprod. 22: 1612-1616

Wakayama S, Suetsugu R, Thuan NV, Ohta H, Kishigami S, and Wakayama T. (2007) Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes. Curr. Biol. 17: R120-R121

Bui HT, Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E, Wakayama T, and Miyano T.(2007) Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes. Reproduction. 133:371-382.

Wakayama S, Hikichi T, Suetsugu R, Sakaide Y, Bui HT, Mizutani E, and Wakayama T. (2007) Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies. Stem Cells. 25:986-993.

Kishigami S, Bui HT, Wakayama S, Tokunaga K, Thuan NV, Hikichi T, Mizutani E, Ohta H, Suetsugu R, Sata T, and Wakayama T. (2007) Success of mouse cloning from an outbred strain by TSA treatment after somatic nuclear transfer. J. Reprod. Dev. 53: 165-170.

Hikichi T, Wakayama S, Mizutani E, Takashima Y, Kishigami S, Van Thuan N, Ohta H, Bui HT, Nishikawa SI, and Wakayama T. (2007) Differentiation Potential of Parthenogenetic Embryonic Stem Cells is Improved by Nuclear Transfer. Stem Cells. 25:46-53.

Singh U, Yu Y, Kalinina E, Konno T, Sun T, Ohta H, Wakayama T, Soares MJ, Hemberger M, and  Fundele RH. (2006) Carboxypeptidase E in the mouse placenta.　Differentiation. 74:648-660.

Mizutani E, Ohta H, Kishigami S, Thuan NV, Hikichi T, Wakayama S, Kosaka M, Sato E, and Wakayama T. Developmental ability of cloned embryos from neural stem cells. Reproduction. 2006 132:849-857.

Wakisaka-Saito N, Kohda T, Inoue K, Ogonuki N, Miki H, Hikichi T, Mizutani E, Wakayama T, Kaneko-Ishino T, Ogura A, and Ishino F. (2006) Chorioallantoic placenta defects in cloned mice. Biochem Biophys Res Commun. 349: 106-114.

Kishigami S, Ohta H, Mizutani E, Wakayama S, Bui HT, Thuan NV, Hikichi T, Suetsugu R, and Wakayama T.(2006) Harmful or Not: Trichostatin A treatment of embryos generated by ICSI or ROSI. Central European Journal of Biology 1: 376-385.

Yoneda A, Okada A, Wakayama T, Ueda J, and Watanabe T. (2006) Analysis of specific factors generating 2-cell block in AKR mouse embryos. Zygote. 14:169-179.

Bui HT, Thuan NV, Wakayama T, and Miyano T. (2006) Chromatin remodeling in somatic cells injected into mature pig oocytes. Reproduction 131: 1037-1049.

Wakayama S, Jakt ML, Suzuki M, Araki R, Hikichi T, Kishigami S, Ohta H, Thuan NV, Mizutani E, Sakaide Y, Senda S, Tanaka S, Okada M, Miyake M, Abe M, Nishikawa SI, Shiota K, and Wakayama T. (2006) Equivalency of Nuclear Transfer-Derived Embryonic Stem Cells to those Derived from Fertilized Mouse Blastocyst. Stem Cells. 24: 2023-2033.

Kishigami S, Hikichi T, Van Thuan N, Ohta H, Wakayama S, Bui HT, Mizutani E, and Wakayama T. (2006) Normal specification of the extraembryonic lineage after somatic nuclear transfer. FEBS Lett. 580:1801-1806

Van Thuan, N., S. Wakayama, S. Kishigami, H. Ohta, T. Hikichi, E. Mizutani, H. T. Bui and T. Wakayama (2006) Injection of Somatic Cell Cytoplasm into Oocytes Before ICSI Impairs Full-Term Development and Increases Placental Weight in Mice, Biol Reprod: 74:865-873

Van Thuan, N., Wakayama, S., Kishigami, S. and Wakayama, T. (2006) Donor Centrosome Regulation of Initial Spindle Formation in Mouse Somatic Cell Nuclear Transfer: Roles of Gamma-Tubulin and Nuclear Mitotic Apparatus Protein 1, Biol Reprod : 74:777-787

Kishigami S, Mizutani E, Ohta H, Hikichi T, Thuan NV, Wakayama S, Bui HT, Wakayama T, Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem Biophys Res Commun 2006 340: 183-189.

Tamada, H., N. V. Thuan, P. Reed, D. Nelson, N. Katoku-Kikyo, J. Wudel, T. Wakayama and N. Kikyo (2006) Chromatin decondensation and nuclear reprogramming by nucleoplasmin, Mol Cell Biol, 26: 1259-1271.

Kishigami S, Thuan NV, Hikichi T, Ohta H, Wakayama S, Mizutani E, Wakayama T. Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids Dev Biol 2006 289: 195-205.

Wakayama, S., E. Mizutani, S. Kishigami, N. V. Thuan, H. Ohta, T. Hikichi, H. T. Bui, M. Miyake and T. Wakayama (2005) Mice Cloned by Nuclear Transfer from Somatic and ntES Cells Derived from the Same Individuals, J Reprod Dev, 51: 765-772.

Singh U, Sun T, Shi W, Schulz R, Nuber UA, Varanou A, Hemberger MC, Elliott RW, Ohta H, Wakayama T, Fundele R. (2005) Expression and Functional Analysis of Genes Deregulated in Mouse Placental Over Growth Models: Car2 and Ncam1. Dev. Dynamics 234:1034-1045

Mizutani E, Ohta H, Kishigami S, Van Thuan N, Hikichi T, Wakayama S, Sato E, Wakayama T Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice. Genesis. 2005 43:34-42.

Fujita, K. Ohta, H. Tsujimura, A. Takao, T. Miyagawa, Y. Takada, S. Matsumiya, K. Wakayama, T. Okuyama, A. (2005) Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia. J Clin Invest. 115:1855-1861

Ohta H, Wakayama T. Generation of Normal Progeny by Intracytoplasmic Sperm Injection Following Grafting of Testicular Tissue from Cloned Mice that Died Postnatally. Biol Reprod. 2005 73:390-395


Wakayama S, Kishigami S, Van Thuan N, Ohta H, Hikichi T, Mizutani E, Yanagimachi R, Wakayama T. (2005) Propagation of an Infertile Hermaphrodite Mouse Lacking Germ Cells, Using Nuclear Transfer and Embryonic Stem Cell Technology. Proc. Natl. Acad. Sci. USA 102:29-33. (Cover article of issue)

Wakayama S, Ohta H, Kishigami S, Van Thuan N, Hikichi T, Mizutani E, Miyake M, Wakayama T. (2005) Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell Lines from Different Mouse Strains and Tissues. Biol. Reprod. 72, 932-936.

Hikichi T, Kishigami S, Thuan NV, Ohta H, Mizutani E, Wakayama S, Wakayama T. (2005) Round spermatids stained with mitotracker can be used to produce offspring more simply. Zygote. 2005 13:55-61.

Van Thuan N, Wakayama S, Kishigami S, and Wakayama T (2005) New Preservation Method for Mouse Spermatozoa Without Freezing. Biol. Reprod. 72, 444?450.

Mochida K, Wakayama T, Takano K, Noguchi Y, Yamamoto Y, Suzuki O, Matsuda J, and Ogura A (2005) Birth of Offspring after Transfer of Mongolian Gerbil (Meriones unguiculatus) Embryos Cryopreserved by Vitrification. Mol. Reprod. Dev. 70: 464-470.

Kishigami S, Thuan NV, Wakayama S, Hikichi T, Wakayama T. (2004) A new separation method of the round-spermatid cytoplasm before ROSI in mouse. Zygote 12: 321-327

Wakayama S, Thuan NV, Kishigami S, Ohta H, Mizutani E, Hikichi T, Miyake M, Wakayama T. (2004) Production of offspring from one-day-old oocytes stored at room temperature. J. Reprod. Dev. 50:627-637.

Senda S, Wakayama T, Yamazaki Y, Ohgane J, Hattori N, Tanaka S, Yanagimachi R, Shiota K. (2004) Skewed X-inactivation in cloned mice. Biochem Biophys Res Commun. 321:38-44.

Ohta H., and Wakayama T. (2004) Full-term development of offspring using ectopically produced haploid germ cells from fetal male germ cells. J. Reprod. Dev. 50:429-437.

Ohta H, Wakayama T, Nishimune Y. (2004) Commitment of Fetal Male Germ Cells to Spermatogonial Stem Cells During Mouse Embryonic Development. Biol Reprod. 70: 1286-1291

Satoshi Kishigami, Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama. (2004) Similar time restriction for ICSI and ROSI into activated oocytes for efficient offspring production Biol Reprod. 70:1863-1869

Lanza R, Moore MA, Wakayama T, Perry AC, Shieh JH, Hendrikx J, Leri A, Chimenti S, Monsen A, Nurzynska D, West MD, Kajstura J, Anversa P. (2004) Regeneration of the Infarcted Heart With Stem Cells Derived by Nuclear Transplantation. Circulation Research. 94:820-827

Ohgane J, Wakayama T, Senda S, Yamazaki Y, Inoue K, Ogura A, Marh J, Tanaka S, Yanagimachi R, Shiota K. (2004) The Sall3 locus is an epigenetic hotspot of aberrant DNA methylation associated with placentomegaly of cloned mice. Genes Cells.;9:253-260

Yanagimachi R., Wakayama T., Kishikawa H., Fimia GM., Monaco L. and Sassone-Corsi P. (2004) Production of fertile offspring from genetically infertile male mice. Proc. Natl. Acad. Sci. USA 101, 1691-1695.

Singh U, Fohn LE, Wakayama T, Ohgane J, Steinhoff C, Lipkowitz B, Schulz R, Orth A, Ropers HH, Behringer RR, Tanaka S, Shiota K, Yanagimachi R, Nuber UA, Fundele R. (2004) Different molecular mechanisms underlie placental overgrowth phenotypes caused by interspecies hybridization, cloning, and Esx1 mutation. Developmental Dynamics. 230, 149-164.

Wakayama S, Cibelli JB, Wakayama T (2003) Effect of timing of the removal of oocyte chromosomes before or after injection of somatic nucleus on development of NT embryos. Cloning and stem cells 5: 181-189.



Reviews

Wakayama T. (2011)Development of novel intracytoplasmic sperm injection and somatic cell nuclear transfer techniques for animal reproduction. Anim Sci J. 82:8-16.

Wakayama S, Mizutani E, Wakayama T. (2010) Production of cloned mice from somatic cells, ES cells, and frozen bodies. Methods Enzymol. 476:151-69.

Thuan NV, Kishigami S and Wakayama T. How to improve the success rate of mouse cloning technology. (2010) J. Reprod. Dev. 56: 20-30.

Wakayama S, Wakayama T. (2010) Improvement of mouse cloning using nuclear transfer-derived embryonic stem cells and/or histone deacetylase inhibitor. Int J Dev Biol. 54:1641-8.

Wakayama S, Kishigami S, Wakayama T. (2009).
Cloning of ES cells and mice by nuclear transfer. Methods Mol Biol. 2009;530:251-65.

Kishigami S, Wakayama T.(2009). Somatic cell nuclear transfer in the mouse. Methods Mol Biol. 518:207-218.

Wakayama S.,  Cummins J.M. and Wakayama T.(2008) Nuclear reprogramming to produce cloned mice and ES cells from somatic cells. RBMOnline 16:545-552.

Kishigami S, Wakayama S, Hosoi Y, Iritani A, and Wakayama T. (2008) Somatic cell nuclear transfer: Infinite reproduction of a unique diploid genome. Exp Cell Res. 314: 1945-1950

Wakayama T. (2007) Production of Cloned Mice and ES Cells from Adult Somatic Cells by Nuclear Transfer: How to Improve Cloning Efficiency?  J Reprod Dev 53: 13-26


Yang X, Smith SL, Tian XC, Lewin HA, Renard JP, Wakayama T.(2007) Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning.
Nat Genet. 39:295-302.

Tamashiro KL, Sakai RR, Yamazaki Y, Wakayama T, Yanagimachi R. (2007) Developmental, behavioral, and physiological phenotype of cloned mice. Adv Exp Med Biol. 591:72-83

Kishigami S, Wakayama S, Thuan NV, Ohta H, Mizutani E, Hikichi T, Bui HT, Balbach S, Ogura A, Boiani M and  Wakayama T. (2006) Production of cloned mice by somatic cellnuclear transfer. Nature Protocols 1: 125-138.

Wakayama T. (2006) Establishment of nuclear transfer embryonic stem cell lines from adult somatic cells by nuclear transfer and its application. Ernst Schering Res Found Workshop. 60: 111-123.

Wakayama T. (2006) Establishment of nuclear transfer embryonic stem cell lines from adult somatic cells by nuclear transfer and its application. Ernst Schering Res Found Workshop. 60: 111-123.

Kishigami S. Wakayama S. Thuan NV, Wakayama T.(2006) Cloned mice and ES cell establishment from adult somatic cell Human Cell 19:2-10

Wakayama S. Wakayama T. (2005) Embryonic stem cell lines from somatic cell nuclei via nuclear transfer. J. Mamm. Ova. Res. 22: 152-158.

Wakayama T. (2004) On the road to therapeutic cloning. Nature Biotechnology. 22: 399-400

Tamashiro KL, Wakayama T, Yamazaki Y, Akutsu H, Woods SC, Kondo S, Yanagimachi R, Sakai RR.　 (2003) Phenotype of Cloned Mice: Development, Behavior, and Physiology.　Exp Biol Med. 228:1193-1200

Wakayama T. (2003) Cloned mice and embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Oncology Research. 13:309-314.

Japanese(日本語総説）

若山清香、李羽中、若山照彦「生殖工学を用いた新たな動物繁殖技術」　実験医学　2012 30: 58-64

李羽中、小野哲男、若山照彦「精子の室温保存法」産婦人科治療　2008 96:395-399

若山清香、引地貴亮、若山照彦　「核移植によるES細胞」実験医学　2008 26:47-52

若山清香、岸上哲士、若山照彦　「体細胞核移植クローンとクローンES細胞の樹立」蛋白質核酸酵素　2007、52:2197-2202


若山清香、若山照彦　「体外受精に失敗した卵子を用いたマウスの体細胞クローンES細胞の樹立」メディカルバイオ　2007、


Thuan NV. Bui HT. 若山照彦　核移植の現状と将来：クローン技術とES細胞　蛋白質核酸酵素 2006, 51: 1768-1774


岸上哲士、若山清香、若山照彦　「体細胞クローン技術の新たな展開」蛋白質核酸酵素増刊　2006、


若山清香、若山照彦　体細胞クローン技術で作られた胚性幹細胞　医学の歩み　2006,217：524-528


若山照彦　体細胞核からES細胞を作り出す技術　学術月報　2006,59：258-262


引地貴亮，岸上哲士，若山照彦　体細胞から作られたES細胞の正常性　―クローン技術は再生医療に利用できるのか　実験医学増刊　2006、24：94-98


水谷英二、若山照彦　クローン技術の発展と現状　腎と透析　2005,59:459-463


若山照彦　哺乳動物の体細胞クローン　哺乳動物卵子学会2005, 22:49-58


大田浩　若山照彦　体細胞核移植による初期発生能の再獲得　実験医学2005, 23:707-711


若山照彦　マウスntES細胞株の作成　Medical Science Digest 2004, 30: 402-405


若山照彦　クローン技術の発展と再生医学　最新医学 2003, 58:537-544


若山照彦　発生過程におけるゲノム・リプログラミング　実験医学 2003, 21: 1058-1512


若山照彦　核の初期化機構とクローン技術の応用　関西実験動物研究会会報 2002, 23:79-82


若山照彦　クローン動物と体細胞由来ES細胞　細胞工学 2002, 21:844-847


若山照彦　クローンマウスと再プログラム化　実験医学 2001, 19: 1487-1493


若山照彦　クローンマウスがもたらす新しい発生生物学　遺伝子医学 2000, 4:232-233


若山照彦　体細胞とES細胞からのクローンマウス　遺伝子医学 2000, 4: 236-240


若山照彦　胚性幹細胞からのクローンマウス　血液・免疫・腫瘍 BIC Forum 2000, 5:386-391


若山照彦　核移植によるマウスのクローン　蛋白質　核酸　酵素 2000, 45: 2005-2014


若山照彦　クローンマウスにかける夢　実験医学 2000, 18:1434-1436


若山照彦　マウスのクローン　遺伝　1999, 53:27-32


若山照彦　成体体細胞からのクローンマウスの作出　Molecular Medicine 1998, 35:1280-1287


若山照彦　マウスICSIマニュアル　日本胚移植学雑誌 1998, 19:199-203



若山照彦　クローンマウス誕生！　実験医学 1998, 16:1921-1923