Optimized conditions for the supplementation of human-induced pluripotent stem cell cultures with a GSK-3 inhibitor during embryoid body formation with the aim of inducing differentiation into mesodermal and cardiac lineage. Yoda K, Ohnuki Y, Masui S, Kurosawa H, J Biosci Bioeng 129, 371-378 (2020).

Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions. Yoda K, Ohnuki Y, Kurosawa H, J Biosci Bioeng 127, 381-387 (2019).

Srf destabilizes cellular identity by suppressing cell-type-specific gene expression programs. Ikeda T, Hikichi T, Miura H, Shibata H, Mitsunaga K, Yamada Y, Woltjen K, Miyamoto K, Watanabe A, Hiratani I, Yamada Y, Yamamoto T, Hotta A, Okita K, Masui S, Nat Commun 9, 1387 (2018).

ROCK inhibitor Y-27632 used during cell aggregate formation adversely affects the early cardiac differentiation on human iPS cells. Ohnuki Y, Kondo T, Kurosawa H, Tissue Cult Res Commun 36, 21-29 (2017).

OVOL2 maintains the transcriptional program of human corneal epithelium by suppressing epithelial-to-mesenchymal transition. Kitazawa K, Hikichi T, Nakamura T, Mitsunaga K, Tanaka A, Nakamura M, Yamakawa T, Furukawa S, Takasaka M, Goshima N, Watanabe A, Okita K, Kawasaki S, Ueno M, Kinoshita S, Masui S, Cell Rep 15, 1359-1368 (2016).

Effective Rho-associated protein kinase inhibitor treatment to dissociate human iPS cells for suspension culture to form embryoid body-like cell aggregates. Horiguchi A, Yazaki K, Aoyagi M, Ohnuki Y, Kurosawa H, J Biosci Bioeng 118, 588-592 (2014).

Transcription factors interfering with dedifferentiation induce cell type-specific transcriptional profiles. Hikichi T, Matoba R, Ikeda T, Watanabe A, Yamamoto T, Yoshitake S, Tamura- Nakano M, Kimura T, Kamon M, Shimura M, Kawakami K, Okuda A, Okochi H, Inoue T, Suzuki A, Masui S, Proc Natl Acad Sci USA 110, 6412-6417 (2013).

Effect of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells: Optimization of culture conditions for the formation of well-controlled embryoid bodies. Ohnuki, Y, Kurosawa, H, J Biosci Bioeng, 115, 571-574 (2013).

Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation. Mochizuki H, Ohnuki Y, and Kurosawa H, J Biosc Bioeng, 111, 92-97 (2011).

Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MSH, Niwa H, Nat Cell Biol 9, 625-635 (2007).

Characterization of embryoid bodies of mouse embryonic stem cells formed under various culture conditions and estimation of differentiation status of such bodies. Koike M, Sakaki S, Amano Y, and Kurosawa H,  J Biosci Bioeng, 104, 294-299 (2007).査読有

A simple method for forming embryoid body from mouse embryonic stem cells. Kurosawa H, Imamura T, Koike M, Sasaki K, Amano Y,  J Biosci Bioeng, 96, 409-411 (2003).